Claudio Vinegoni, Ph.D. | Multispectral photoacoustic imaging of fluorochromes in small animals

Opt. Lett.
Multispectral photoacoustic imaging of fluorochromes in small animals

Razansky, D., Vinegoni, C., and Ntziachristos^#, V.

Optics Letters 2007

ABS URL PMID DOI CITE HTML PDF

Fluorochromes have become essential reporter molecules in biological research. We show that the depth-resolved distribution of fluorochromes in small animals can be imaged with 25 fmol sensitivity and 150 Am spatial resolution by means of multispectral photoacoustic imaging. The major advantage of the multispectral approach is the sensitive differentiation of chromophores and fluorochromes of interest based on self-reference measurements, as evidenced in this study by resolving a commonly used fluorochrome (Alexa Fluor 750) in mouse. The suggested method is well suited for enhancing visualization of functional and molecular information in vivo and longitudinally. (c) 2007 Optical Society of America.
@article{2007-OL, author= {Razansky, D. and Vinegoni, C. and Ntziachristos<sup>#</sup>, V.}, title= {Multispectral photoacoustic imaging of fluorochromes in small animals}, journal= {Optics Letters}, alternatejournal = {Opt. Lett.}, year = {2007}, volume = {32}, number = {19}, pages = {2891-2893}, issn = {0146-9592}, doi = {10.1364/ol.32.002891}, pmid= {17909608} }
17909608

10.1364/ol.32.002891

Abstract

"Fluorochromes have become essential reporter molecules in biological research. We show that the depth-resolved distribution of fluorochromes in small animals can be imaged with 25 fmol sensitivity and 150 Am spatial resolution by means of multispectral photoacoustic imaging. The major advantage of the multispectral approach is the sensitive differentiation of chromophores and fluorochromes of interest based on self-reference measurements, as evidenced in this study by resolving a commonly used fluorochrome (Alexa Fluor 750) in mouse. The suggested method is well suited for enhancing visualization of functional and molecular information in vivo and longitudinally. (c) 2007 Optical Society of America."

Full citation

For attribution in academic contexts, please cite this work as:

Razansky, D., Vinegoni, C., & Ntziachristos^#, V. (2007). Multispectral photoacoustic imaging of fluorochromes in small animals. Optics Letters, 32(19), 2891–2893. https://doi.org/10.1364/ol.32.002891