In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography

Nature Methods

  1. Nat. Methods
    In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography
    Vinegoni#†, C., Pitsouli†, C., Razansky†, D., Perrimon, N., and Ntziachristos, V.
    Nature Methods 2008
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Abstract

"We report a technique for fluorescence tomography that operates beyond the penetration limits of tissue-sectioning fluorescence microscopy. The method uses multi-projection illumination and photon transport description in opaque tissues. We demonstrate whole-body three-dimensional visualization of the morphogenesis of GFP-expressing salivary glands and wing imaginal discs in living Drosophila melanogaster pupae in vivo and over time."

Full citation

For attribution in academic contexts, please cite this work as:
Vinegoni#†, C., Pitsouli†, C., Razansky†, D., Perrimon, N., & Ntziachristos, V. (2008). In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography. Nature Methods, 5(1), 45–47. https://doi.org/10.1038/nmeth1149



Vinegoni#†, C., Pitsouli†, C., Razansky†, D., Perrimon, N., & Ntziachristos, V. (2008). In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography. Nature Methods, 5(1), 45–47. https://doi.org/10.1038/nmeth1149