Claudio Vinegoni, Ph.D. | Multispectral opto-acoustic tomography of deep-seated fluorescent proteins in vivo

Nat. Photonics
Multispectral opto-acoustic tomography of deep-seated fluorescent proteins in vivo

Razansky^#, D., Distel, M., Vinegoni, C., Ma, R., Perrimon, N., Koster, R. W., and Ntziachristos^#, V.

Nature Photonics 2009

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Fluorescent proteins have become essential reporter molecules for studying life at the cellular and sub-cellular level, re-defining the ways in which we investigate biology. However, because of intense light scattering, most organisms and tissues remain inaccessible to current fluorescence microscopy techniques at depths beyond several hundred micrometres. We describe a multispectral opto-acoustic tomography technique capable of high-resolution visualization of fluorescent proteins deep within highly light-scattering living organisms. The method uses multiwavelength illumination over multiple projections combined with selective-plane opto-acoustic detection for artifact-free data collection. Accurate image reconstruction is enabled by making use of wavelength-dependent light propagation models in tissue. By performing whole-body imaging of two biologically important and optically diffuse model organisms, Drosophila melanogaster pupae and adult zebrafish, we demonstrate the facility to resolve tissue-specific expression of eGFP and mCherrry fluorescent proteins for precise morphological and functional observations in vivo.
@article{2009-NATPHOT, author= {Razansky<sup>#</sup>, D. and Distel, M. and Vinegoni, C. and Ma, R. and Perrimon, N. and Koster, R. W. and Ntziachristos<sup>#</sup>, V.}, title= {Multispectral opto-acoustic tomography of deep-seated fluorescent proteins in vivo}, journal= {Nature Photonics}, alternatejournal = {Nat. Photonics}, year = {2009}, volume = {3}, number = {7}, pages = {412-417}, issn = {1749-4885}, doi = {10.1038/nphoton.2009.98}, }
10.1038/nphoton.2009.98

Abstract

"Fluorescent proteins have become essential reporter molecules for studying life at the cellular and sub-cellular level, re-defining the ways in which we investigate biology. However, because of intense light scattering, most organisms and tissues remain inaccessible to current fluorescence microscopy techniques at depths beyond several hundred micrometres. We describe a multispectral opto-acoustic tomography technique capable of high-resolution visualization of fluorescent proteins deep within highly light-scattering living organisms. The method uses multiwavelength illumination over multiple projections combined with selective-plane opto-acoustic detection for artifact-free data collection. Accurate image reconstruction is enabled by making use of wavelength-dependent light propagation models in tissue. By performing whole-body imaging of two biologically important and optically diffuse model organisms, Drosophila melanogaster pupae and adult zebrafish, we demonstrate the facility to resolve tissue-specific expression of eGFP and mCherrry fluorescent proteins for precise morphological and functional observations in vivo."

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For attribution in academic contexts, please cite this work as:

Razansky^#, D., Distel, M., Vinegoni, C., Ma, R., Perrimon, N., Koster, R. W., & Ntziachristos^#, V. (2009). Multispectral opto-acoustic tomography of deep-seated fluorescent proteins in vivo. Nature Photonics, 3(7), 412–417. https://doi.org/10.1038/nphoton.2009.98