Claudio Vinegoni, Ph.D. | In-vivo two-photon imaging of the honey bee antennal lobe

Biomed. Opt. Exp.
In-vivo two-photon imaging of the honey bee antennal lobe

Haase, A., Rigosi, E., Trona, F., Anfora, G., Vallortigara, G., Antolini, R., and Vinegoni^#, C.

Biomedical Optics Express 2011

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Due to the honey bee’s importance as a simple neural model, there is a great need for new functional imaging modalities. Herein we report on the development and new finding of a combined two-photon microscope with a synchronized odor stimulus platform for in-vivo functional and morphological imaging of the honey bee’s olfactory system focusing on its primary centers, the antennal lobes (ALs). Our imaging platform allows for simultaneously obtaining both morphological measurements of the AL’s functional units, the glomeruli, and in-vivo calcium recording of their neural activities. By applying external odor stimuli to the bee’s antennae, we were able to record the characteristic glomerular odor response maps. Compared to previous works where conventional fluorescenc microscopy was used, our approach has been demonstrated to offer all the advantages of multi-photon imaging, providing substantial enhancement in both spatial and temporal resolutions while minimizing photo-damages. In addition, compared to previous full-fiel microscopy calcium recordings, a four-fold improvement in the functional signal has been achieved. Finally, the multi-photon associated extended penetration depth allows for functional imaging of profound glomeruli. (C) 2010 Optical Society of America
@article{2010-BOEX, author= {Haase, A. and Rigosi, E. and Trona, F. and Anfora, G. and Vallortigara, G. and Antolini, R. and Vinegoni<sup>#</sup>, C.}, title= {In-vivo two-photon imaging of the honey bee antennal lobe}, journal= {Biomedical Optics Express}, alternatejournal = {Biomed. Opt. Exp.}, year = {2011}, volume = {2}, number = {1}, pages = {131-138}, issn = {2156-7085}, doi = {10.1364/boe.2.000131}, pmcid = {PMC3028488}, pmid= {21326643} }
21326643

PMC3028488

10.1364/boe.2.000131

Abstract

"Due to the honey bee's importance as a simple neural model, there is a great need for new functional imaging modalities. Herein we report on the development and new finding of a combined two-photon microscope with a synchronized odor stimulus platform for in-vivo functional and morphological imaging of the honey bee's olfactory system focusing on its primary centers, the antennal lobes (ALs). Our imaging platform allows for simultaneously obtaining both morphological measurements of the AL's functional units, the glomeruli, and in-vivo calcium recording of their neural activities. By applying external odor stimuli to the bee's antennae, we were able to record the characteristic glomerular odor response maps. Compared to previous works where conventional fluorescenc microscopy was used, our approach has been demonstrated to offer all the advantages of multi-photon imaging, providing substantial enhancement in both spatial and temporal resolutions while minimizing photo-damages. In addition, compared to previous full-fiel microscopy calcium recordings, a four-fold improvement in the functional signal has been achieved. Finally, the multi-photon associated extended penetration depth allows for functional imaging of profound glomeruli. (C) 2010 Optical Society of America"

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For attribution in academic contexts, please cite this work as:

Haase, A., Rigosi, E., Trona, F., Anfora, G., Vallortigara, G., Antolini, R., & Vinegoni^#, C. (2011). In-vivo two-photon imaging of the honey bee antennal lobe. Biomedical Optics Express, 2(1), 131–138. https://doi.org/10.1364/boe.2.000131