Claudio Vinegoni, Ph.D. | Imaging of molecular probe activity with Born-normalized fluorescence optical projection tomography

Opt. Lett.
Imaging of molecular probe activity with Born-normalized fluorescence optical projection tomography

Vinegoni^#, C., Fumene Feruglio, P., Cortez-Retamozo, V., Razansky, D., Medoff, B. D., Ntziachristos, V., Sbarbati, A., Pittet, M., and Weissleder, R.

Optics Letters 2010

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Optical projection tomography is a new ex vivo imaging technique that allows imaging of whole organs in three dimensions at high spatial resolutions. In this Letter we demonstrate its capability to tomographically visualize molecular activity in whole organs of mice. In particular, eosinophil activity in asthmatic lungs is resolved using a Born-normalized fluorescence optical projection tomography and employing a near-IR molecular probe. The possibility to achieve molecularly sensitive imaging contrast in optical projection tomography by means of targeted and activatable imaging reporter agents adds a new range of capabilities for investigating molecular signatures of pathophysiological processes and a wide variety of diseases and their development. (C) 2010 Optical Society of America
@article{2010-OL, author= {Vinegoni<sup>#</sup>, C. and Fumene Feruglio, P. and Cortez-Retamozo, V. and Razansky, D. and Medoff, B. D. and Ntziachristos, V. and Sbarbati, A. and Pittet, M. and Weissleder, R.}, title= {Imaging of molecular probe activity with Born-normalized fluorescence optical projection tomography}, journal= {Optics Letters}, alternatejournal = {Opt. Lett.}, year = {2010}, volume = {35}, number = {7}, pages = {1088-1090}, issn = {0146-9592}, doi = {10.1364/ol.35.001088}, pmcid = {PMC2900933}, pmid= {20364226} }
20364226

PMC2900933

10.1364/ol.35.001088

Abstract

"Optical projection tomography is a new ex vivo imaging technique that allows imaging of whole organs in three dimensions at high spatial resolutions. In this Letter we demonstrate its capability to tomographically visualize molecular activity in whole organs of mice. In particular, eosinophil activity in asthmatic lungs is resolved using a Born-normalized fluorescence optical projection tomography and employing a near-IR molecular probe. The possibility to achieve molecularly sensitive imaging contrast in optical projection tomography by means of targeted and activatable imaging reporter agents adds a new range of capabilities for investigating molecular signatures of pathophysiological processes and a wide variety of diseases and their development. (C) 2010 Optical Society of America"

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Vinegoni^#, C., Fumene Feruglio, P., Cortez-Retamozo, V., Razansky, D., Medoff, B. D., Ntziachristos, V., Sbarbati, A., Pittet, M., & Weissleder, R. (2010). Imaging of molecular probe activity with Born-normalized fluorescence optical projection tomography. Optics Letters, 35(7), 1088–1090. https://doi.org/10.1364/ol.35.001088