Claudio Vinegoni, Ph.D. | Hybrid PET-optical imaging using targeted probes

P.N.A.S.
Hybrid PET-optical imaging using targeted probes

Nahrendorf^†#, M., Keliher^†, E., Marinelli, B., Waterman, P., Fumene Feruglio, P., Fexon, L., Pivovarov, M., Swirski, F. K., Pittet, M. J., Vinegoni, C., and Weissleder^#, R.

Proceedings of the National Academy of Sciences of the United States of America 2010

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Fusion imaging of radionuclide-based molecular (PET) and structural data [x-ray computed tomography (CT)] has been firmly established. Here we show that optical measurements [fluorescence-mediated tomography (FMT)] show exquisite congruence to radionuclide measurements and that information can be seamlessly integrated and visualized. Using biocompatible nanoparticles as a generic platform (containing a F-18 isotope and a far red fluorochrome), we show good correlations between FMT and PET in probe concentration (r(2) > 0.99) and spatial signal distribution (r(2) > 0.85). Using a mouse model of cancer and different imaging probes to measure tumoral proteases, macrophage content and integrin expression simultaneously, we demonstrate the distinct tumoral locations of probes in multiple channels in vivo. The findings also suggest that FMT can serve as a surrogate modality for the screening and development of radionuclide-based imaging agents.
@article{2010-PNAS, author= {Nahrendorf†#, M. and Keliher†, E. and Marinelli, B. and Waterman, P. and Fumene Feruglio, P. and Fexon, L. and Pivovarov, M. and Swirski, F. K. and Pittet, M. J. and Vinegoni, C. and Weissleder#, R.}, title= {Hybrid PET-optical imaging using targeted probes}, journal= {Proceedings of the National Academy of Sciences of the United States of America}, alternatejournal = {P.N.A.S.}, year = {2010}, volume = {107}, number = {17}, pages = {7910-7915}, issn = {0027-8424}, doi = {10.1073/pnas.0915163107}, pmcid = {PMC2867879}, pmid= {20385821} }
20385821

PMC2867879

10.1073/pnas.0915163107

Abstract

"Fusion imaging of radionuclide-based molecular (PET) and structural data [x-ray computed tomography (CT)] has been firmly established. Here we show that optical measurements [fluorescence-mediated tomography (FMT)] show exquisite congruence to radionuclide measurements and that information can be seamlessly integrated and visualized. Using biocompatible nanoparticles as a generic platform (containing a F-18 isotope and a far red fluorochrome), we show good correlations between FMT and PET in probe concentration (r(2) > 0.99) and spatial signal distribution (r(2) > 0.85). Using a mouse model of cancer and different imaging probes to measure tumoral proteases, macrophage content and integrin expression simultaneously, we demonstrate the distinct tumoral locations of probes in multiple channels in vivo. The findings also suggest that FMT can serve as a surrogate modality for the screening and development of radionuclide-based imaging agents."

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For attribution in academic contexts, please cite this work as:

Nahrendorf^†#, M., Keliher^†, E., Marinelli, B., Waterman, P., Fumene Feruglio, P., Fexon, L., Pivovarov, M., Swirski, F. K., Pittet, M. J., Vinegoni, C., & Weissleder^#, R. (2010). Hybrid PET-optical imaging using targeted probes. Proceedings of the National Academy of Sciences of the United States of America, 107(17), 7910–7915. https://doi.org/10.1073/pnas.0915163107