Claudio Vinegoni, Ph.D. | Motion compensation using a suctioning stabilizer for intravital microscopy

Intravital
Motion compensation using a suctioning stabilizer for intravital microscopy

Vinegoni^#†, C., Lee^†, S., Gorbatov, R., and Weissleder, R.

Intravital 2012

ABS PMID DOI PMCID CITE HTML PDF

Motion artifacts continue to present a major challenge to single cell imaging in cardiothoracic organs such as the beating heart, blood vessels or lung. In this study, we present a new water-immersion suctioning stabilizer that enables minimally invasive intravital fluorescence microscopy using water-based stick objectives. The stabilizer works by reducing major motion excursions and can be used in conjunction with both prospective or retrospective gating approaches. We show that the new approach offers cellular resolution in the beating murine heart without perturbing normal physiology. In addition, because this technique allows multiple areas to be easily probed, it offers the opportunity for wide area coverage at high resolution.
@article{2012-INTRVTL, author= {Vinegoni<sup>#†</sup>, C. and Lee<sup>†</sup>, S. and Gorbatov, R. and Weissleder, R.}, title= {Motion compensation using a suctioning stabilizer for intravital microscopy}, journal= {Intravital}, alternatejournal = {Intravital}, year = {2012}, volume = {1}, pages = {115}, issn = {1083-3668}, doi = {10.4161/intv.23017}, pmcid = {PMC3786172}, pmid= {24086796} }
24086796

PMC3786172

10.4161/intv.23017

Abstract

"Motion artifacts continue to present a major challenge to single cell imaging in cardiothoracic organs such as the beating heart, blood vessels or lung. In this study, we present a new water-immersion suctioning stabilizer that enables minimally invasive intravital fluorescence microscopy using water-based stick objectives. The stabilizer works by reducing major motion excursions and can be used in conjunction with both prospective or retrospective gating approaches. We show that the new approach offers cellular resolution in the beating murine heart without perturbing normal physiology. In addition, because this technique allows multiple areas to be easily probed, it offers the opportunity for wide area coverage at high resolution."

Full citation

For attribution in academic contexts, please cite this work as:

Vinegoni^#†, C., Lee^†, S., Gorbatov, R., & Weissleder, R. (2012). Motion compensation using a suctioning stabilizer for intravital microscopy. Intravital, 1, 115. https://doi.org/10.4161/intv.23017