Claudio Vinegoni, Ph.D. | Real-time in vivo imaging of the beating mouse heart at microscopic resolution

Nat. Commun.
Real-time in vivo imaging of the beating mouse heart at microscopic resolution

Lee^†, S., Vinegoni^#†, C., Fumene Feruglio, P., Fexon, L., Gorbatov, R., Pivoravov, M., Sbarbati, A., Nahrendorf, M., and Weissleder, R.

Nature Communications 2012

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Real-time imaging of moving organs and tissues at microscopic resolutions represents a major challenge in studying the complex biology of live animals. Here we present a technique based on a novel stabilizer setup combined with a gating acquisition algorithm for the imaging of a beating murine heart at the single-cell level. The method allows serial in vivo fluorescence imaging of the beating heart in live mice in both confocal and nonlinear modes over the course of several hours. We demonstrate the utility of this technique for in vivo optical sectioning and dual-channel time-lapse fluorescence imaging of cardiac ischaemia. The generic method could be adapted to other moving organs and thus broadly facilitate in vivo microscopic investigations.
@article{2012-NC, author= {Lee<sup>†</sup>, S. and Vinegoni<sup>#†</sup>, C. and Fumene Feruglio, P. and Fexon, L. and Gorbatov, R. and Pivoravov, M. and Sbarbati, A. and Nahrendorf, M. and Weissleder, R.}, title= {Real-time in vivo imaging of the beating mouse heart at microscopic resolution}, journal= {Nature Communications}, alternatejournal = {Nat. Commun.}, year = {2012}, volume = {3}, pages = {8}, issn = {2041-1723}, doi = {10.1038/ncomms2060}, pmcid = {PMC3622400}, pmid= {22968700} }
22968700

PMC3622400

10.1038/ncomms2060

Abstract

"Real-time imaging of moving organs and tissues at microscopic resolutions represents a major challenge in studying the complex biology of live animals. Here we present a technique based on a novel stabilizer setup combined with a gating acquisition algorithm for the imaging of a beating murine heart at the single-cell level. The method allows serial in vivo fluorescence imaging of the beating heart in live mice in both confocal and nonlinear modes over the course of several hours. We demonstrate the utility of this technique for in vivo optical sectioning and dual-channel time-lapse fluorescence imaging of cardiac ischaemia. The generic method could be adapted to other moving organs and thus broadly facilitate in vivo microscopic investigations."

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For attribution in academic contexts, please cite this work as:

Lee^†, S., Vinegoni^#†, C., Fumene Feruglio, P., Fexon, L., Gorbatov, R., Pivoravov, M., Sbarbati, A., Nahrendorf, M., & Weissleder, R. (2012). Real-time in vivo imaging of the beating mouse heart at microscopic resolution. Nature Communications, 3, 8. https://doi.org/10.1038/ncomms2060