Claudio Vinegoni, Ph.D. | Imaging Therapeutic PARP Inhibition In Vivo through Bioorthogonally Developed Companion Imaging Agents

Neoplasia
Imaging Therapeutic PARP Inhibition In Vivo through Bioorthogonally Developed Companion Imaging Agents

Reiner, T., Lacy, J., Keliher, E. J., Yang, K. S., Ullal, A., Kohler, R. H., Vinegoni, C., and Weissleder^#, R.

Neoplasia 2012

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A number of small-molecule poly (ADP-ribose) polymerase (PARP) inhibitors are currently undergoing advanced clinical trials. Determining the distribution and target inhibitory activity of these drugs in individual subjects, however, has proven problematic. Here, we used a PARP agent for positron emission tomography-computed tomography (PET-CT) imaging (F-18-BO), which we developed based on the Olaparib scaffold using rapid bioorthogonal conjugation chemistries. We show that the bioorthogonal F-18 modification of the parent molecule is simple, highly efficient, and well tolerated, resulting in a half maximal inhibitory concentration (IC50) of 17.9 +/- 1.1 nM. Intravital imaging showed ubiquitous distribution of the drug and uptake into cancer cells, with ultimate localization within the nucleus, all of which were inhibitable. Whole-body PET-CT imaging showed tumoral uptake of the drug, which decreased significantly, after a daily dose of Olaparib. Standard F-18-fludeoxyglucose imaging, however, failed to detect such therapy-induced changes. This research represents a step toward developing a more generic approach for the rapid codevelopment of companion imaging agents based on small-molecule therapeutic inhibitors.
@article{2012-NEOPLASIA, author= {Reiner, T. and Lacy, J. and Keliher, E. J. and Yang, K. S. and Ullal, A. and Kohler, R. H. and Vinegoni, C. and Weissleder<sup>#</sup>, R.}, title= {Imaging Therapeutic PARP Inhibition In Vivo through Bioorthogonally Developed Companion Imaging Agents}, journal= {Neoplasia}, alternatejournal = {Neoplasia}, year = {2012}, volume = {14}, number = {3}, pages = {169-+}, issn = {1476-5586}, doi = {10.1593/neo.12414}, pmcid = {PMC3323895}, pmid= {22496617} }
22496617

PMC3323895

10.1593/neo.12414

Abstract

"A number of small-molecule poly (ADP-ribose) polymerase (PARP) inhibitors are currently undergoing advanced clinical trials. Determining the distribution and target inhibitory activity of these drugs in individual subjects, however, has proven problematic. Here, we used a PARP agent for positron emission tomography-computed tomography (PET-CT) imaging (F-18-BO), which we developed based on the Olaparib scaffold using rapid bioorthogonal conjugation chemistries. We show that the bioorthogonal F-18 modification of the parent molecule is simple, highly efficient, and well tolerated, resulting in a half maximal inhibitory concentration (IC50) of 17.9 +/- 1.1 nM. Intravital imaging showed ubiquitous distribution of the drug and uptake into cancer cells, with ultimate localization within the nucleus, all of which were inhibitable. Whole-body PET-CT imaging showed tumoral uptake of the drug, which decreased significantly, after a daily dose of Olaparib. Standard F-18-fludeoxyglucose imaging, however, failed to detect such therapy-induced changes. This research represents a step toward developing a more generic approach for the rapid codevelopment of companion imaging agents based on small-molecule therapeutic inhibitors."

Full citation

For attribution in academic contexts, please cite this work as:

Reiner, T., Lacy, J., Keliher, E. J., Yang, K. S., Ullal, A., Kohler, R. H., Vinegoni, C., & Weissleder^#, R. (2012). Imaging Therapeutic PARP Inhibition In Vivo through Bioorthogonally Developed Companion Imaging Agents. Neoplasia, 14(3), 169-+. https://doi.org/10.1593/neo.12414