Claudio Vinegoni, Ph.D. | Implantable microenvironments to attract hematopoietic stem/cancer cells

P.N.A.S.
Implantable microenvironments to attract hematopoietic stem/cancer cells

Lee, J., Li, M., Milwid, J., Dunham, J., Vinegoni, C., Gorbatov, R., Iwamoto, Y., Wang, F. J., Shen, K. Y., Hatfield, K., Enger, M., Shafiee, S., McCormack, E., Ebert, B. L., Weissleder, R., Yarmush, M. L., and Parekkadan^#, B.

Proceedings of the National Academy of Sciences of the United States of America 2012

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The environments that harbor hematopoietic stem and progenitor cells are critical to explore for a better understanding of hematopoiesis during health and disease. These compartments often are inaccessible for controlled and rapid experimentation, thus limiting studies to the evaluation of conventional cell culture and transgenic animal models. Here we describe the manufacture and image-guided monitoring of an engineered microenvironment with user-defined properties that recruits hematopoietic progenitors into the implant. Using intravital imaging and fluorescence molecular tomography, we show in real time that the cell homing and retention process is efficient and durable for short-and long-term engraftment studies. Our results indicate that bone marrow stromal cells, precoated on the implant, accelerate the formation of new sinusoidal blood vessels with vascular integrity at the micro-capillary level that enhances the recruitment hematopoietic progenitor cells to the site. This implantable construct can serve as a tool enabling the study of hematopoiesis.
@article{2012-PNAS, author= {Lee, J. and Li, M. and Milwid, J. and Dunham, J. and Vinegoni, C. and Gorbatov, R. and Iwamoto, Y. and Wang, F. J. and Shen, K. Y. and Hatfield, K. and Enger, M. and Shafiee, S. and McCormack, E. and Ebert, B. L. and Weissleder, R. and Yarmush, M. L. and Parekkadan<sup>#</sup>, B.}, title= {Implantable microenvironments to attract hematopoietic stem/cancer cells}, journal= {Proceedings of the National Academy of Sciences of the United States of America}, alternatejournal = {P.N.A.S.}, year = {2012}, volume = {109}, number = {48}, pages = {19638-19643}, issn = {0027-8424}, doi = {10.1073/pnas.1208384109}, pmcid = {PMC3786172}, pmid= {24086796} }
24086796

PMC3786172

10.1073/pnas.1208384109

Abstract

"The environments that harbor hematopoietic stem and progenitor cells are critical to explore for a better understanding of hematopoiesis during health and disease. These compartments often are inaccessible for controlled and rapid experimentation, thus limiting studies to the evaluation of conventional cell culture and transgenic animal models. Here we describe the manufacture and image-guided monitoring of an engineered microenvironment with user-defined properties that recruits hematopoietic progenitors into the implant. Using intravital imaging and fluorescence molecular tomography, we show in real time that the cell homing and retention process is efficient and durable for short-and long-term engraftment studies. Our results indicate that bone marrow stromal cells, precoated on the implant, accelerate the formation of new sinusoidal blood vessels with vascular integrity at the micro-capillary level that enhances the recruitment hematopoietic progenitor cells to the site. This implantable construct can serve as a tool enabling the study of hematopoiesis."

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Lee, J., Li, M., Milwid, J., Dunham, J., Vinegoni, C., Gorbatov, R., Iwamoto, Y., Wang, F. J., Shen, K. Y., Hatfield, K., Enger, M., Shafiee, S., McCormack, E., Ebert, B. L., Weissleder, R., Yarmush, M. L., & Parekkadan^#, B. (2012). Implantable microenvironments to attract hematopoietic stem/cancer cells. Proceedings of the National Academy of Sciences of the United States of America, 109(48), 19638–19643. https://doi.org/10.1073/pnas.1208384109