Claudio Vinegoni, Ph.D. | Sequential average segmented microscopy for high signal-to-noise ratio motion-artifact-free in vivo heart imaging

Biomed. Opt. Exp.
Sequential average segmented microscopy for high signal-to-noise ratio motion-artifact-free in vivo heart imaging

Vinegoni^#†, C., Lee^†, S., Fumene Feruglio, P., Marzola, P., Nahrendorf, M., and Weissleder, R.

Biomedical Optics Express 2013

ABS URL PMID DOI PMCID CITE HTML PDF

In vivo imaging is often severely compromised by cardiovascular and respiratory motion. Highly successful motion compensation techniques have been developed for clinical imaging (e.g. magnetic resonance imaging) but the use of more advanced techniques for intravital microscopy is largely unexplored. Here, we implement a sequential cardiorespiratory gating scheme (SCG) for averaged microscopy. We show that SCG is very efficient in eliminating motion artifacts, is highly practical, enables high signal-to-noise ratio (SNR) in vivo imaging, and yields large field of views. The technique is particularly useful for high-speed data acquisition or for imaging scenarios where the fluorescence signal is not significantly above noise or background levels. (c) 2013 Optical Society of America
@article{2013-BOE, author= {Vinegoni<sup>#†</sup>, C. and Lee<sup>†</sup>, S. and Fumene Feruglio, P. and Marzola, P. and Nahrendorf, M. and Weissleder, R.}, title= {Sequential average segmented microscopy for high signal-to-noise ratio motion-artifact-free in vivo heart imaging}, journal= {Biomedical Optics Express}, alternatejournal = {Biomed. Opt. Exp.}, year = {2013}, volume = {4}, number = {10}, pages = {2095-2106}, issn = {2156-7085}, doi = {10.1364/boe.4.002095}, pmcid = {PMC3799669}, pmid= {24156067} }
24156067

PMC3799669

10.1364/boe.4.002095

Abstract

"In vivo imaging is often severely compromised by cardiovascular and respiratory motion. Highly successful motion compensation techniques have been developed for clinical imaging (e.g. magnetic resonance imaging) but the use of more advanced techniques for intravital microscopy is largely unexplored. Here, we implement a sequential cardiorespiratory gating scheme (SCG) for averaged microscopy. We show that SCG is very efficient in eliminating motion artifacts, is highly practical, enables high signal-to-noise ratio (SNR) in vivo imaging, and yields large field of views. The technique is particularly useful for high-speed data acquisition or for imaging scenarios where the fluorescence signal is not significantly above noise or background levels. (c) 2013 Optical Society of America"

Full citation

For attribution in academic contexts, please cite this work as:

Vinegoni^#†, C., Lee^†, S., Fumene Feruglio, P., Marzola, P., Nahrendorf, M., & Weissleder, R. (2013). Sequential average segmented microscopy for high signal-to-noise ratio motion-artifact-free in vivo heart imaging. Biomedical Optics Express, 4(10), 2095–2106. https://doi.org/10.1364/boe.4.002095