Steady state anisotropy two-photon microscopy resolves multiple, spectrally similar fluorophores, enabling in vivo multilabel imaging

Optics Letters

  1. Opt. Lett.
    Steady state anisotropy two-photon microscopy resolves multiple, spectrally similar fluorophores, enabling in vivo multilabel imaging
    Dubach†, J. M., Vinegoni#†, C., and Weissleder, R.
    Optics Letters 2014
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Abstract

"The use of spectrally distinguishable fluorescent dyes enables imaging of multiple targets. However, in two-photon microscopy, the number of fluorescent labels with distinct emission spectra that can be effectively excited and resolved is constrained by the confined tuning range of the excitation laser and the broad and overlapping nature of fluorophore two-photon absorption spectra. This limitation effectively reduces the number of available imaging channels. Here, we demonstrate that two-photon steady state anisotropy imaging (2PSSA) offers the capability to resolve otherwise unresolvable fluorescent tracers both in live cells and in mouse tumor models. This approach expands the number of biological targets that can be imaged simultaneously, increasing the total amount of information that can be obtained through imaging. (C) 2014 Optical Society of America"

Full citation

For attribution in academic contexts, please cite this work as:
Dubach†, J. M., Vinegoni#†, C., & Weissleder, R. (2014). Steady state anisotropy two-photon microscopy resolves multiple, spectrally similar fluorophores, enabling in vivo multilabel imaging. Optics Letters, 39(15), 4482–4485. https://doi.org/10.1364/ol.39.004482



Dubach†, J. M., Vinegoni#†, C., & Weissleder, R. (2014). Steady state anisotropy two-photon microscopy resolves multiple, spectrally similar fluorophores, enabling in vivo multilabel imaging. Optics Letters, 39(15), 4482–4485. https://doi.org/10.1364/ol.39.004482