Claudio Vinegoni, Ph.D. | Intravital imaging of cardiac function at the single-cell level

P.N.A.S.
Intravital imaging of cardiac function at the single-cell level

Aguirre, A. D., Vinegoni^#, C., Sebas, M., and Weissleder^#, R.

Proceedings of the National Academy of Sciences of the United States of America 2014

ABS URL PMID DOI PMCID CITE HTML PDF

Knowledge of cardiomyocyte biology is limited by the lack of methods to interrogate single-cell physiology in vivo. Here we show that contracting myocytes can indeed be imaged with optical microscopy at high temporal and spatial resolution in the beating murine heart, allowing visualization of individual sarcomeres and measurement of the single cardiomyocyte contractile cycle. Collectively, this has been enabled by efficient tissue stabilization, a prospective real-time cardiac gating approach, an image processing algorithm for motion-artifact-free imaging throughout the cardiac cycle, and a fluorescent membrane staining protocol. Quantification of cardiomyocyte contractile function in vivo opens many possibilities for investigating myocardial disease and therapeutic intervention at the cellular level.
@article{2014-PNAS, author= {Aguirre, A. D. and Vinegoni<sup>#</sup>, C. and Sebas, M. and Weissleder<sup>#</sup>, R.}, title= {Intravital imaging of cardiac function at the single-cell level}, journal= {Proceedings of the National Academy of Sciences of the United States of America}, alternatejournal = {P.N.A.S.}, year = {2014}, volume = {111}, number = {31}, pages = {11257-11262}, issn = {0027-8424}, doi = {10.1073/pnas.1401316111}, pmcid = {PMC4128110}, pmid= {25053815} }
25053815

PMC4128110

10.1073/pnas.1401316111

Abstract

"Knowledge of cardiomyocyte biology is limited by the lack of methods to interrogate single-cell physiology in vivo. Here we show that contracting myocytes can indeed be imaged with optical microscopy at high temporal and spatial resolution in the beating murine heart, allowing visualization of individual sarcomeres and measurement of the single cardiomyocyte contractile cycle. Collectively, this has been enabled by efficient tissue stabilization, a prospective real-time cardiac gating approach, an image processing algorithm for motion-artifact-free imaging throughout the cardiac cycle, and a fluorescent membrane staining protocol. Quantification of cardiomyocyte contractile function in vivo opens many possibilities for investigating myocardial disease and therapeutic intervention at the cellular level."

Full citation

For attribution in academic contexts, please cite this work as:

Aguirre, A. D., Vinegoni^#, C., Sebas, M., & Weissleder^#, R. (2014). Intravital imaging of cardiac function at the single-cell level. Proceedings of the National Academy of Sciences of the United States of America, 111(31), 11257–11262. https://doi.org/10.1073/pnas.1401316111