Claudio Vinegoni, Ph.D. | Rapid, high efficiency isolation of pancreatic beta-cells

Sci. Rep.
Rapid, high efficiency isolation of pancreatic beta-cells

Clardy, S. M., Mohan, J. F., Vinegoni, C., Keliher, E. J., Iwamoto, Y., Benoist, C., Mathis, D., and Weissleder^#, R.

Scientific Reports 2015

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The ability to isolate pure pancreatic beta-cells would greatly aid multiple areas of diabetes research. We developed a fluorescent exendin-4-like neopeptide conjugate for the rapid purification and isolation of functional mouse pancreatic beta-cells. By targeting the glucagon-like peptide-1 receptor with the fluorescent conjugate, beta-cells could be quickly isolated by flow cytometry and were >99% insulin positive. These studies were confirmed by immunostaining, microscopy and gene expression profiling on isolated cells. Gene expression profiling studies of cytofluorometrically sorted beta-cells from 4 and 12 week old NOD mice provided new insights into the genetic programs at play of different stages of type-1 diabetes development. The described isolation method should have broad applicability to the beta-cell field.
@article{2015-SREP, author= {Clardy, S. M. and Mohan, J. F. and Vinegoni, C. and Keliher, E. J. and Iwamoto, Y. and Benoist, C. and Mathis, D. and Weissleder<sup>#</sup>, R.}, title= {Rapid, high efficiency isolation of pancreatic beta-cells}, journal= {Scientific Reports}, alternatejournal = {Sci. Rep.}, year = {2015}, volume = {5}, pages = {9}, issn = {2045-2322}, doi = {10.1038/srep13681}, pmcid = {PMC4557033}, pmid= {26330153} }
26330153

PMC4557033

10.1038/srep13681

Abstract

"The ability to isolate pure pancreatic beta-cells would greatly aid multiple areas of diabetes research. We developed a fluorescent exendin-4-like neopeptide conjugate for the rapid purification and isolation of functional mouse pancreatic beta-cells. By targeting the glucagon-like peptide-1 receptor with the fluorescent conjugate, beta-cells could be quickly isolated by flow cytometry and were >99% insulin positive. These studies were confirmed by immunostaining, microscopy and gene expression profiling on isolated cells. Gene expression profiling studies of cytofluorometrically sorted beta-cells from 4 and 12 week old NOD mice provided new insights into the genetic programs at play of different stages of type-1 diabetes development. The described isolation method should have broad applicability to the beta-cell field."

Full citation

For attribution in academic contexts, please cite this work as:

Clardy, S. M., Mohan, J. F., Vinegoni, C., Keliher, E. J., Iwamoto, Y., Benoist, C., Mathis, D., & Weissleder^#, R. (2015). Rapid, high efficiency isolation of pancreatic beta-cells. Scientific Reports, 5, 9. https://doi.org/10.1038/srep13681