Claudio Vinegoni, Ph.D. | Bone marrow endothelial dysfunction promotes myeloid cell expansion in cardiovascular disease

Nat. Card. Res.
Bone marrow endothelial dysfunction promotes myeloid cell expansion in cardiovascular disease

Rohde, D., Vandoorne, K., Lee, I. H., Grune, J., Zhang, S., McAlpine, C. S., Schloss, M. J., Nayar, R., Courties, G., Frodermann, V., Wojtkiewicz, G., Honold, L., Chen, Q., Schmidt, S., Iwamoto, Y., Sun, Y., Cremer, S., Hoyer, F. F., Iborra-Egea, O., Muñoz-Guijosa, C., Ji, F., Zhou, G., Adams, R. H., Wythe, J. D., Hidalgo, J., Watanabe, H., Jung, Y., Laan, A. M., Piek, J. J., Kfoury, Y., Désogère, P. A., Vinegoni, C., Dutta, P., Sadreyev, R. I., Caravan, P., Bayes-Genis, A., Libby, P., Scadden, D. T., Lin, C. P., Naxerova, K., Swirski, F. K., and Nahrendorf^#, M.

Nature Cardiovascular Research 2022

ABS PMID DOI PMCID CITE HTML PDF

Abnormal hematopoiesis advances cardiovascular disease by generating excess inflammatory leukocytes that attack the arteries and the heart. The bone marrow niche regulates hematopoietic stem cell proliferation and hence the systemic leukocyte pool, but whether cardiovascular disease affects the hematopoietic organ’s microvasculature is unknown. Here we show that hypertension, atherosclerosis and myocardial infarction (MI) instigate endothelial dysfunction, leakage, vascular fibrosis and angiogenesis in the bone marrow, altogether leading to overproduction of inflammatory myeloid cells and systemic leukocytosis. Limiting angiogenesis with endothelial deletion of Vegfr2 (encoding vascular endothelial growth factor (VEGF) receptor 2) curbed emergency hematopoiesis after MI. We noted that bone marrow endothelial cells assumed inflammatory transcriptional phenotypes in all examined stages of cardiovascular disease. Endothelial deletion of Il6 or Vcan (encoding versican), genes shown to be highly expressed in mice with atherosclerosis or MI, reduced hematopoiesis and systemic myeloid cell numbers in these conditions. Our findings establish that cardiovascular disease remodels the vascular bone marrow niche, stimulating hematopoiesis and production of inflammatory leukocytes.
@article{2022-NCR-1, author= {Rohde, D. and Vandoorne, K. and Lee, I. H. and Grune, J. and Zhang, S. and McAlpine, C. S. and Schloss, M. J. and Nayar, R. and Courties, G. and Frodermann, V. and Wojtkiewicz, G. and Honold, L. and Chen, Q. and Schmidt, S. and Iwamoto, Y. and Sun, Y. and Cremer, S. and Hoyer, F. F. and Iborra-Egea, O. and Muñoz-Guijosa, C. and Ji, F. and Zhou, G. and Adams, R. H. and Wythe, J. D. and Hidalgo, J. and Watanabe, H. and Jung, Y. and van der Laan, A. M. and Piek, J. J. and Kfoury, Y. and Désogère, P. A. and Vinegoni, C. and Dutta, P. and Sadreyev, R. I. and Caravan, P. and Bayes-Genis, A. and Libby, P. and Scadden, D. T. and Lin, C. P. and Naxerova, K. and Swirski, F. K. and Nahrendorf<sup>#</sup>, M.}, title= {Bone marrow endothelial dysfunction promotes myeloid cell expansion in cardiovascular disease}, journal= {Nature Cardiovascular Research}, alternatejournal = {Nat. Card. Res.}, year = {2022}, volume = {1}, number = {1}, pages = {28-44}, issn = {2731-0590}, doi = {10.1038/s44161-021-00002-8}, pmcid = {PMC9216333}, pmid= {35747128} }
35747128

PMC9216333

10.1038/s44161-021-00002-8

Abstract

"Abnormal hematopoiesis advances cardiovascular disease by generating excess inflammatory leukocytes that attack the arteries and the heart. The bone marrow niche regulates hematopoietic stem cell proliferation and hence the systemic leukocyte pool, but whether cardiovascular disease affects the hematopoietic organ’s microvasculature is unknown. Here we show that hypertension, atherosclerosis and myocardial infarction (MI) instigate endothelial dysfunction, leakage, vascular fibrosis and angiogenesis in the bone marrow, altogether leading to overproduction of inflammatory myeloid cells and systemic leukocytosis. Limiting angiogenesis with endothelial deletion of Vegfr2 (encoding vascular endothelial growth factor (VEGF) receptor 2) curbed emergency hematopoiesis after MI. We noted that bone marrow endothelial cells assumed inflammatory transcriptional phenotypes in all examined stages of cardiovascular disease. Endothelial deletion of Il6 or Vcan (encoding versican), genes shown to be highly expressed in mice with atherosclerosis or MI, reduced hematopoiesis and systemic myeloid cell numbers in these conditions. Our findings establish that cardiovascular disease remodels the vascular bone marrow niche, stimulating hematopoiesis and production of inflammatory leukocytes."

Full citation

For attribution in academic contexts, please cite this work as:

Rohde, D., Vandoorne, K., Lee, I. H., Grune, J., Zhang, S., McAlpine, C. S., Schloss, M. J., Nayar, R., Courties, G., Frodermann, V., Wojtkiewicz, G., Honold, L., Chen, Q., Schmidt, S., Iwamoto, Y., Sun, Y., Cremer, S., Hoyer, F. F., Iborra-Egea, O., … Nahrendorf^#, M. (2022). Bone marrow endothelial dysfunction promotes myeloid cell expansion in cardiovascular disease. Nature Cardiovascular Research, 1(1), 28–44. https://doi.org/10.1038/s44161-021-00002-8