Claudio Vinegoni, Ph.D. | Advanced Motion Compensation Methods for Intravital Optical Microscopy

IEEE J.S.T.Q.E.
Advanced Motion Compensation Methods for Intravital Optical Microscopy

Vinegoni^#†, C., Lee^†, S., Fumene Feruglio, P., and Weissleder, R.

Ieee Journal of Selected Topics in Quantum Electronics 2014

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Intravital microscopy has emerged in the recent decade as an indispensible imaging modality for the study of the microdynamics of biological processes in live animals. Technical advancements in imaging techniques and hardware components, combined with the development of novel targeted probes and new mice models, have enabled us to address long-standing questions in several biology areas such as oncology, cell biology, immunology, and neuroscience. As the instrument resolution has increased, physiological motion activities have become a major obstacle that prevents imaging live animals at resolutions analogue to the ones obtained in vitro. Motion compensation techniques aim at reducing this gap and can effectively increase the in vivo resolution. This paper provides a technical review of some of the latest developments in motion compensation methods, providing organ specific solutions.
@article{2014-IEEE, author= {Vinegoni<sup>#†</sup>, C. and Lee<sup>†</sup>, S. and Fumene Feruglio, P. and Weissleder, R.}, title= {Advanced Motion Compensation Methods for Intravital Optical Microscopy}, journal= {Ieee Journal of Selected Topics in Quantum Electronics}, alternatejournal = {IEEE J.S.T.Q.E.}, year = {2014}, volume = {20}, number = {2}, pages = {9}, issn = {1077-260X}, doi = {10.1109/jstqe.2013.2279314}, pmcid = {PMC3832946}, pmid= {24273405} }
24273405

PMC3832946

10.1109/jstqe.2013.2279314

Abstract

"Intravital microscopy has emerged in the recent decade as an indispensible imaging modality for the study of the microdynamics of biological processes in live animals. Technical advancements in imaging techniques and hardware components, combined with the development of novel targeted probes and new mice models, have enabled us to address long-standing questions in several biology areas such as oncology, cell biology, immunology, and neuroscience. As the instrument resolution has increased, physiological motion activities have become a major obstacle that prevents imaging live animals at resolutions analogue to the ones obtained in vitro. Motion compensation techniques aim at reducing this gap and can effectively increase the in vivo resolution. This paper provides a technical review of some of the latest developments in motion compensation methods, providing organ specific solutions."

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Vinegoni^#†, C., Lee^†, S., Fumene Feruglio, P., & Weissleder, R. (2014). Advanced Motion Compensation Methods for Intravital Optical Microscopy. Ieee Journal of Selected Topics in Quantum Electronics, 20(2), 9. https://doi.org/10.1109/jstqe.2013.2279314