Claudio Vinegoni, Ph.D. | Motion characterization scheme to minimize motion artifacts in intravital microscopy

J. Biomed. Opt.
Motion characterization scheme to minimize motion artifacts in intravital microscopy

Lee, S., Courties, G., Nahrendorf, M., Weissleder, R., and Vinegoni^#, C.

Journal of Biomedical Optics 2017

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Respiratory- and cardiac-induced motion artifacts pose a major challenge for in vivo optical imaging, limiting the temporal and spatial imaging resolution in fluorescence laser scanning microscopy. Here, we present an imaging platform developed for in vivo characterization of physiologically induced axial motion. The motion characterization system can be straightforwardly implemented on any conventional laser scanning microscope and can be used to evaluate the effectiveness of different motion stabilization schemes. This method is particularly useful to improve the design of novel tissue stabilizers and to facilitate stabilizer positioning in real time, therefore facilitating optimal tissue immobilization and minimizing motion induced artifacts. (C) 2017 Society of Photo-Optical Instrumentation Engineers (SPIE).
@article{2017-JBO, author= {Lee, S. and Courties, G. and Nahrendorf, M. and Weissleder, R. and Vinegoni<sup>#</sup>, C.}, title= {Motion characterization scheme to minimize motion artifacts in intravital microscopy}, journal= {Journal of Biomedical Optics}, alternatejournal = {J. Biomed. Opt.}, year = {2017}, volume = {22}, number = {3}, pages = {7}, issn = {1083-3668}, doi = {10.1117/1.Jbo.22.3.036005}, pmcid = {PMC5333764}, pmid= {28253383} }
28253383

PMC5333764

10.1117/1.Jbo.22.3.036005

Abstract

"Respiratory- and cardiac-induced motion artifacts pose a major challenge for in vivo optical imaging, limiting the temporal and spatial imaging resolution in fluorescence laser scanning microscopy. Here, we present an imaging platform developed for in vivo characterization of physiologically induced axial motion. The motion characterization system can be straightforwardly implemented on any conventional laser scanning microscope and can be used to evaluate the effectiveness of different motion stabilization schemes. This method is particularly useful to improve the design of novel tissue stabilizers and to facilitate stabilizer positioning in real time, therefore facilitating optimal tissue immobilization and minimizing motion induced artifacts. (C) 2017 Society of Photo-Optical Instrumentation Engineers (SPIE)."

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For attribution in academic contexts, please cite this work as:

Lee, S., Courties, G., Nahrendorf, M., Weissleder, R., & Vinegoni^#, C. (2017). Motion characterization scheme to minimize motion artifacts in intravital microscopy. Journal of Biomedical Optics, 22(3), 7. https://doi.org/10.1117/1.Jbo.22.3.036005